Zhao Ting Ting
China-Japan Friendship Hospital, P R China
Title: The glycosaminoglycan chain of decorin plays an important role in AMPK signaling
Biography
Biography: Zhao Ting Ting
Abstract
Diabetic nephropathy (DN) is one of the major microvascular complications of diabetes. Energy metabolism is altered under diabetic conditions. Decorin is a multifunctional small leucine-rich proteoglycan involved in the autophagy and energetic homeostasis. We investigated the role of decorin and its glycosaminoglycan chain in DN. Both decorin and phosphoralated AMPK were highly expressed in the kidney of an accelerated type 1 DN induced by streptozotocin in uninephrectomized Wistar rats at week 20. In high glucose cultured HK2 cell, glycosaminoglycan-free decorin, generated by mutating Ser4 of the mature protein core into Ala (DCN-S4A), showed more significant role in promoting of AMPK phosphoralation and LC3 II compared with the decorin proteoglycan. These data provides clues that the glycosaminoglycan chain of decorin has a reducing effect on activation of AMPK signaling pathway in DN.