Julian M Menter
Morehouse School of Medicine, USA
Title: Effect of temperature on photochemical and thermal changes in calf-skin collagen solutions at physiological pH
Biography
Biography: Julian M Menter
Abstract
Mammalian collagens exhibit weak intrinsic age- and history-dependent UV fluorescence that. UV radiation can cause longer wavelength fluorescent oxidative bands. The fluorescence of both unirradiated and UVC – irradiated acid-soluble calf skin collagen also reflects considerable autoxidation. We have been studying the ground-and excited state behavior of type I acid – soluble collagens from commercial calf skin and from acid-extracted hairless mouse skin as judged by their temperature and wavelength dependence of their fluorescence properties. As the age-related oxidation products destabilize the overall collagen supramolecular structure, we wondered whether the surrounding extracellular matrix affects the ground- and excited state behavior of the collagen polymer in situ. Added sodium hyaluronate (2:1 ratio) shows little or no effect on fluorescence behavior or Arrhenius – type plots. This latter result may indicate that there is little physical interaction between the collagen telopeptide and HA domains in vitro.
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