Achuth Padmanabhan
Yale University, USA
Title: Tracking cancer progression through monitoring the dynamic serum phospho-proteome
Biography
Biography: Achuth Padmanabhan
Abstract
Serum proteome has been shown to reflect pathological changes in tissues. Changes in serum proteomic pattern during carcinogenesis have been exploited successfully to serve as diagnostic and prognostic markers. Protein Kinase A catalytic subunit α has been shown to be secreted into the serum as ecto-kinase and the ecto-PKA level is significantly higher in the serum of prostate cancer patients. Here, I describe the utility of the Reverse In-gel Kinase Assay coupled with LC-MS/MS to profile potential serum substrates of PKA and in following their phosphorylation status during disease progression (1-4). The sensitivity and linearity of this assay will help identify potential biomarkers, which can be used to track the progression of prostate cancer. This strategy can be adapted and extended to track the progression of other cancers and pathological conditions.