Glycobiology World Congress

Biography

Biography: Shigeomi Horito

Abstract

Tetraspanin is four times transmembrane protein which constructs a super molecular complex with ganglioside GM3 and CD81 to regulate cell proliferation. Cancer proliferation is also regulate this super molecular complex. It is exciting challenge to elucidate the role of ganglioside GM3 in the super molecular complex. Guofei Son et al reported reconstruction of transmembrane protein aquaporine Z into lipid bilayer by interaction of nickel chelate and histidine tag. We tried to reconstruct tetraspanin by interaction of only natural compounds. GM3/dipalmitoylphosphatigylcholine/dioreoilphosphatigylcholine(1/9/9) lipid-monolayer indicate 2 types domains (0.9 nm high and 2.1 nm high). Hetero-bilayer indicates 2 types domains (6.7 nm high and 12.5 nm high), which is given tetraspanin to indicate 3 types domains (6.1 nm high, 11.7 nm high and 15.6 nm). The highest domain indicates the reconstruction of tetraspanin into the lipid-bilayer.