Glycobiology World Congress

Biography

Biography: Maria O. Longas

Abstract

Alzheimer’s Disease Rats were developed by Taconic Biosciences, MPA; development took 5 weeks. After this time, each of the 6 Alzheimer’s Disease rats was injected with NaH (3 mg/3 mL sterilized phosphate buffer saline, SPBS); 3 grams of NaH/gram of Rat body mass was used for injection. The controls were normal Rats, 6 of which were injected with 3mL of SPBS per gram of Rat body mass. After 5 weeks, all Rats were guillotined; the heads were removed and stored at -35oC. Cells were then cultured following conditions described for human Alzheimer’s Disease cells (Longas, MO, et al, unpublished) and carried out in 6-well-tissue-culture-dishes, each containing 4 mL of the following media: Astrocyte Basal Medium from Lonza adjusted to 15% (w/w) fetal bovine serum and fortified with L-Glutamine (5.0 mL), Ascorbic Acid (0.5 mL), Epidermal Growth Factors (1.25 mL), Insulin (0.5mL) and Gentamicin Sulfate Amphotencin-ß (0.5 mL). Cells grew in these media at 37oC, under 5% CO2 for 7 days. They were then stained with Congo Red. Figure IIIR displays Alzheimer’s Disease Hippocampus cells which show plenty of β-Amyloid tangles, while Figure IVR has cells from the Normal Hippocampus with no tangle formation. NaH is, therefore, effective to inhibit β-Amyloid tangle formation in Alzheimer’s Disease.