3^rd Glycobiology World Congress June 26-28, 2017 London, UK

Biography:

Tung-Kung Wu completed his PhD degree in Biophysics from the Johns Hopkins University and postdoctoral studies in Chemistry Department from Stanford University. He is appointed professor in the Department of Biological Science and Technology at the National Chiao Tung University. His research interests include interdisciplinary fields of bioorganic chemistry, chemical biology, and medicinal chemistry. His current research interests mainly focus on the structure-reactivity relationships of glycoenzymes and mode of action of steryl glycosides as drug candidates. 

Abstract:

Steryl glycosides consist of a sterol/steroid aglycone and one or more sugar moieties linked through an ether or ester bond. Diverse biological activities, including anti-inflammatory, anti-fungal, anti-microbial, anti-parasitic, and anti-tumor activities, and properties such as cell membrane modification, regulation of host defenses against pathogens, lipid metabolism, and developmental events, have been attributed to this family of natural products. Development of structural diverse steryl glycosides may hold potential to identify candidates for new drug development and pharmaceutical applications. In this study, we functionally express different glycosyltransferases for in vitro synthesis of steryl glycosides with a- or b-configuration specificity. The synthesized steryl glycosides were investigated for their anticancer activity. Interestingly, several steryl glycosides show dose-dependent depression of cell viability and enhanced drug effectiveness on MCF-7 breast cancer cells. The structure-reactivity relationships of these glycosyltransferases on substrate‒donor specificities and mode of action of steryl glycosides on anticancer activity will be discussed.

Biography:

Xue-Wei Liu, China Agricultural University (BSc & MSc 1996), University of Southern California (PhD 2000), Procter & Gamble (Research Scientist, 2000-2002), Chugai Pharma USA (Senior Research Scientist, 2002-2003), Caltech (Postdoc, 2003-2005). Currently Assoc/Professor at Nanyang Technological University, Singapore. Research field: carbohydrate chemistry, natural products, and glycoproteins in addressing problems of medicinal and biochemical significance.

Abstract:

Glycopeptides and glycoproteins play vital roles in a wide array of biological activities including cell-cell adhesion, cell differentiation, cell growth and tumour metastasis. In addition, a host of major illnesses such as autoimmune diseases, infectious diseases and cancer are caused by aberrant protein glycosylation. Consequently, the development of new glycopeptides-based vaccines, diagnostics and therapeutics has gained significant interests, stimulating the study of glycopeptide synthesis. Here, a practical approach towards N-glycopeptide synthesis using an auxiliary-mediated dual native chemical ligation (NCL) is presented. The first NCL connects an N-linked glycosyl auxiliary to the thioester side chain of an N-terminal aspartate oligopeptide. This intermediate undergoes a second NCL with a C-terminal thioester peptide. Mild deprotection provides the desired N-glycopeptide. The flexibility and systematic control of our method clearly have significant advantages over preceding ones and present a major step forward in glycoscience. Taking into consideration of the previous approaches, our method offers an attractive and practical procedure to N-glycopeptide that does not require cysteine residue, allowing for straightforward glycosylation and ligation at aspartic acid terminal. The auxiliary-linked glycosyl donor can be obtained from readily available glycosyl azide. Our method can leverage modern techniques such as intein-mediated ligation or recombinant expression to gain access to peptides of virtually any length and order. In addition, quick access to polymeric peptidoglycans will also be presented.

Biography:

She received her BSC degree in Biochemistry, Faculty of Science from Ege University in 2006. She is currently doing her master thesis about “Glycan Analysis of Monoclonal Antibodies” and she will be graduated at 2017 summer. At the same time she is working as a scientific researcher at the Center for Drug Research & Development and Pharmacokinetic Applications (ARGEFAR) since 2008. Her professional experience includes usage of HPLC, LC MS, MALDI TOF MS techniques. Her research interest covers physicochemical characterization of biosimilar drugs and biopharmaceutics.

Abstract:

Monoclonal antibodies (MAbs) are increasingly being used for the treatment of cancer or other autoimmune diseases. Correct glycan structure of MAbs is essential for functional and biological activity of the related Mab such as identification of antigens triggered by immune system cells, regulation of the signaling activities and physicochemical properties of produced therapeutics etc. Therefore, rapid monitoring of glycan structure is very critical for therapeutic drug production, biosimilar drug production and their quality control processes.

The principal of this study is the characterization of glycan structure of MAbs. In our study, glycan structure of trastuzumab, used as a drug for the treatment of breast cancer, was characterized. N-glycans were enzymatically released from Trastuzumab protein structure by trypsin digestion and PNGase F (Peptide:N-Glycosidase F) cleavage.  Moreover, special buffer exchange and Solid Phase extraction procedures were used for the clean-up purposes prior to MALDI-TOF-MS (Matrix Assisted Laser Desorption/Ionization-Time of Flight- Mass Spectrometry) detection.

After designing appropriate sample treatment and optimization, GO, GOF, G1, G1F, GOF-GN  glycans were clearly detected by MALDI-Q-IT-TOF MS without any labelling or using any additional chromatographic separation methods.

The current talk deals with the theoretical and experimental aspects of MALDI-Q-IT-TOF MS for the study of glycans on monoclonal antibodies. Sample preparation procedures and tips for MALDI-TOF-MS measurements will be addressed and the original data obtained from MALDI-Q-IT-TOF MS will be presented.

Biography:

Minghua Hu is R&D Lead Engineer of Infinitus (China) Company Ltd that is engaged in Chinese herbal plantations,R&D, production, sales and service of TCM health products. She is also the principle members of Joint Laboratory for the Research of Chinese Herbal Polysaccharides-Chinese Academy of Science Shanghai Institute of Materia Medica and Infinitus. She has published more than 20 papers in reputed journals

Abstract:

Fungal polysaccharides have been shown broad spectrum of biological activities, including anti-inflammatory, ant-oxidative and improve immunity. However, oral administration of fungal polysaccharides for rendering the conventional vaccine against influenza virus has been reported rarely. Here, we investigated the potential of fungal polysaccharides enhancing the influenza vaccine efficacy in a mouse model. Mice were immunized with inactivated H1N1 (A/PR8/1934) influenza vaccine combined with oral polysaccharides lentinan, tremellan, pachymaran, and a mixture of the three. The results showed that mice in the polysaccharides/vaccine groups had reduced morbidity, improved viral clearance, and recovered faster than the mice receiving the conventional vaccine only after infection. This effect could be attributed to the increased levels of virus-specific serum antibody IgG and decreased levels of inflammatory cytokine IFN-γ in the lung tissue. Our finding suggests that taking fungal polysaccharides orally might be useful for improving the efficacy of conventional inactive influenza vaccines.

References

1.            Luo Xia,Minghua Hu ,et(2016)Different impacts of polysaccharides from Chinese materia medica on T-cell subsets in immuno-suppressed mice . Prac J Med&Pharm．2016,07:621-624.

2.            Xiangliang Deng, Zhonghua Ma, Xiaoping Lai, Minghua Hu,et(2016)Rhizoma dioscoreae polysaccharide promotes M1 macrophage polarization in mice.IMMUNOLOGICAL JOURNAL 2016,12:1019-1023.

3.            LUO Xia,MA Zhonghua,HU Minghua,et(2016)Effects of compound polysaccharide on cellular immunity in mice.CHINESE JOUï¼²NAL OF FOOD HYGIENE 2016,02:186-191.

4.            REN Wenkang,HU Minghua,YIN Xiquan,et(2016)Effect of Cyclocarya paliurus（Batal.）Ijinsk Aqueous Extracts on Improving Traditional Chinese Medical Symptoms and Immune Function of Type 2 Diabetic Mellitus Rats with Yin Deficiency Generating Intrinsic Heat Syndrome.Journal of Guangzhou University of Traditional Chinese Medicine 2016,06:851-855.

5.            Minghua Hu,et(2012)Comparison of Content of the Water-Soluble Polysaccharide Included in Poria Cocos from Different Habitat and Specification.China Pharmaceuticals 2012,07:10-12.

Biography:

Ting Ting Zhao has completed her PhD at the age of 27 years from University of Chinese Academy of Sciences. She has published 19 papers in the international journals.

Abstract:

Diabetic nephropathy (DN) is one of the major microvascular complications of diabetes. Energy metabolism is altered under diabetic conditions. Decorin is a multifunctional small leucine-rich proteoglycan involved in the autophagy and energetic homeostasis. We investigated the role of decorin and its glycosaminoglycan chain in DN. Both decorin and phosphoralated AMPK were highly expressed in the kidney of an accelerated type 1 DN induced by streptozotocin in uninephrectomized Wistar rats at week 20. In high glucose cultured HK2 cell, glycosaminoglycan-free decorin, generated by mutating Ser4 of the mature protein core into Ala (DCN-S4A), showed more significant role in promoting of AMPK phosphoralation and LC3 II compared with the decorin proteoglycan. These data provides clues that the glycosaminoglycan chain of decorin has a reducing effect on activation of AMPK signaling pathway in DN.